ABSTRACT

A simple, precise, rapid and accurate reverse phase HPLC method has been developed for the determination of Paclitaxel in bulk and its pharmaceutical dosage form. An enable C18G, 250mm, X4.6mm i.d, 5μm particle size column was used with photo diode array UV-Visible detector. The mobile phase consisting of Acetonitrile and water in the ratio of 80:20V/V was used. The flow rate was 1ml/min and the effluent was monitored at 228nm. The retention time of the drug was 3.737 minutes. The method was linear over the concentration range of 30-250μg/ml. the method precision for the determination of assay was below 2% RSD. The percentage recovery of paclitaxel was 99.41 – 100.83%. The validation of method was carried out utilizing ICH guidelines.

Keywords: Paclitaxel, RP- HPLC, Development, Validation.

ABSTRACT

A simple, precise, rapid and accurate reverse phase HPLC method has been developed for the determination of Ceftibuten in bulk and its pharmaceutical dosage form. An enable C18G, 250mmX4.6mm i.d, 5μm particle size column was used with photo diode array UV-Visible detector. The mobile phase consisting of a mixture of phosphate buffer pH3.0 and acetonitrile HPLC grade (35:65) as the mobile phase at a flow rate 1.0 mL/min, the detection was carried out at 228nm. The retention time of the drug was 2.435 minutes. The method was linear over the concentration range of 10-80μg/ml. the limit of detection and limit of quantification were 0.11 and 0.34 respectively. The percentage recovery of Ceftibuten was 99.41 – 100.83%. The validation of method was carried out utilizing ICH guidelines.

Keywords: Ceftibuten, RP- HPLC, Development, Validation.

ABSTRACT

Citrullus colocynthis (L.) a member of Cucurbitaceae family, was collected from the different areas of the Kachchh region. The fruit pulp of Citrullus colocynthis (L.) was subjected to continuous and successive soxhlet extraction with n-Butanol. The extract was dried in vacuum, antimicrobial activity was measured for the same. With the help of GC/MS analysis the compounds responsible for the biological activity were identified.

Keywords: Soxhlet extraction, Citrulluscolocynthis, GC/MS, Antimicrobial activity.

ABSTRACT

Describing the simple, precise and rapid method based on difference absorbance spectroscopic technique for the determination of Ofloxacin (OF) and ornidazole (OZ) in tablet dosage form. The projected method was based upon measuring the difference absorbance of OF at 293.4 nm (λ max of 0.1N HCl) in 0.1N NaOH vs 0.1N HCl solutions and other drug OZ difference absorbance in 0.1 N NaOH vs 0.1 N HCl at 277.9 nm (λ max of 0.1N HCl). The Beer’s law was obeyed in the concentration range of 4-24 ug/mL and 5-30 μg/mL for OF and OZ respectively. The lower limits of detection (LOD) of OF and OZ are 0.31 and 0.35 μg/mL, respectively, while the lower limits of quantification (LOQ) of OF and OZ were 0.92 and 1.04 μg/ mL, respectively. The precision and accuracy of the method was acceptable; the relative standard deviations did not exceed 1.1% (n=6) and recovery was between 98.25% and 100.8% for OF and OZ, respectively. The proposed method was validated and successfully applied for the determination of both drugs in bulk powder, laboratory prepared mixture and commercial dosage forms such as tablets without interference from the commonly encountered excipients and additives.

Keywords: Ofloxacin, Ornidazole, Difference spectroscopy, Beer’s law.

ABSTRACT

Pharmaceutical analysis is one of the most important fields in analytical chemistry. The discovery of new drugs and the on-going update of international regulations for the safety and efficacy of pharmaceutical formulations demand the continuous development of new analytical methods. Inevitably, automation plays an important role, especially when a lot of samples have to be analyzed in the minimum of time. The present study reviews the applications of flow injection (FI) spectrophotometry to the determination of active pharmaceutical ingredients (APIs) in their respective formulations. However, the topic covered in this study is important not only to pharmaceutical analytical scientists. The principles, figures of merit and \"chemistry\" of the presented methods can be of interest to bio-analytical and clinical chemists as well for the analysis of biological samples, to environmental analysts that study the up-to-date demand of the determination of the fate of pharmaceuticals in the environment and even to toxicologists and forensic scientists. This review covers scientific contributions published later than 2000. A variety of FI procedures based on homogeneous (direct UV measurements, colour-forming reactions, metal-drug interactions) and heterogeneous (optical sensors and solid-phase reactors) systems are discussed and also covers on-line sample pre-treatment (solid-phase extraction, liquid-liquid extraction, on-line digestion, etc).

Keywords: Flow injection spectroscopy, Active pharmaceutical ingredients, Bio-analysis.

ABSTRACT

In bioanalysis samples of plasma, serum, tissues, urine and feces are collected. Before analysis these are processed by using various methods. Tissue samples are processed by using mechanical, digestion, or extraction methods. Plasma and serum are processed by using plasma crash, SPE, LLE methods. Urine samples processed by using dilute and shoot’ (D&S) method and solid phase extraction (SPE). In some cases, acid hydrolysis (for basic compounds) or base hydrolysis (for acidic compounds) is used. Feces are dried and made into powder then extracted by adding a suitable solvent mixture. Then centrifuged and further processed as urine samples.

Keywords: Bioanalysis, Plasma, SPE (solid phase extraction), LLE (liquid – liquid extraction).

ABSTRACT

The present review should extensively be discussed on an international level to reach a consensus on the extent of validation experiments and on acceptance criteria for validation parameters of bioanalytical methods in forensic clinical toxicology. The field of pharmacokinetic (PK) studies for registration of pharmaceuticals was discussed previously. Therefore, the most important analytical findings and validation parameters were discussed here 

Keywords: Validation parameters, Calibration model,Limits, Stability.

ABSTRACT

The study was conducted to analyze the phytoconstituents of Tragia brevipes plant leaves. The plant samples were extracted using methanol and water in the ratio 9:1. The phytoconstituents study was done using standard procedures [18, 19 and 20]. From the study Tragia brevipes was found to contain tannins, saponins, terpenoids, flavonoids, cardiac glycoside, phenols, steroidal nucleus and steroidal ring, but alkaloids and steroids were found to be absent in the plant extract.

Keywords: Tragia brevipes, Phytoconstituents, Medicinal herbs.

ABSTRACT

The aerial parts of Merremia tridentata (Linn) Hallier.f.  of Convolvulaceae is used for hair growth promotion in folklore medicine. In the present investigation a detailed pharmacognostical study of aerial parts of Merremia tridentata L. is carried out as a preliminary step to standardize the raw materials for the  purpose of identification of adulterants and substitute from original drug as well as which could be useful to lay down development of Pharmacopoeial monograph. The study includes macroscopical studies, physico- chemical properties, fluorescence analysis, preliminary phytochemical screening and TLC study.

Keywords: Merremia tridentata, Pharmacognostical, Phytochemical and TLC study.